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Two structural features drive the analysis: the N-terminal acetyl group, whose absence shifts mass by 42 Da and is resolved by MS rather than by retention time, and the D-phenylalanine at position 7, which is what confers protease resistance over the native α-MSH sequence — a batch synthesised with the L-enantiomer at that position is chromatographically similar but biologically different, so chiral integrity is part of identity rather than an assumed property of the synthesis

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Nowadays the insulin needles are so short and fine that nearly everyone finds that their insulin injections are far more comfortable than finger pricks